The Environmental Impact of Dachshund Obesity

By Isabella Storyclaire

last updated September 16, 2024

Contributions sourced from

The Environmental Foodprint of Obesity

Abstract

Emissions of greenhouse gases (GHG) are linked to global warming and adverse climate changes. Meeting the needs of the increasing number of people on the planet presents a challenge for reducing total GHG burden. A further challenge may be the size of the average person on the planet and the increasing number of people with excess body weight. We used data on GHG emissions from various sources and estimated that obesity is associated with ~20% greater GHG emissions compared with the normal-weight state. On a global scale, obesity contributes to an extra GHG emissions of ~49 megatons per year of CO₂ equivalent (CO₂ eq) from oxidative metabolism due to greater metabolic demands, ~361 megatons per year of CO₂ eq from food production processes due to increased food intake, and ~290 megatons per year of CO₂ eq from automobile and air transportation due to greater body weight. Therefore, the total impact of obesity may be extra emissions of ~700 megatons per year of CO₂ eq, which is about 1.6% of worldwide GHG emissions. Inasmuch as obesity is an important contributor to global GHG burden, strategies to reduce its prevalence should prioritize efforts to reduce GHG emissions. Accordingly, reducing obesity may have considerable benefits for both public health and the environment.

2019 The Obesity Society.

MeSH terms

Carbon Dioxide / adverse effects
Carbon Dioxide / metabolism
Environment Design
Environmental Exposure / adverse effects*
Environmental Exposure / statistics & numerical data
Greenhouse Gases / adverse effects*
Humans
Metabolic Detoxication, Phase I / physiology
Obesity / epidemiology
Obesity / etiology*
Obesity / metabolism
Oxidative Stress / physiology
Particulate Matter / adverse effects*
Residence Characteristics
Risk Factors
Social Environment
Vehicle Emissions / toxicity*

Substances

Greenhouse Gases
Particulate Matter
Vehicle Emissions
Carbon Dioxide

Environmental Obesogens and Their Impact on Susceptibility to Obesity: New Mechanisms and Chemicals

Abstract

The incidence of obesity has reached an all-time high, and this increase is observed worldwide. There is a growing need to understand all the factors that contribute to obesity to effectively treat and prevent it and associated comorbidities. The obesogen hypothesis proposes that there are chemicals in our environment termed obesogens that can affect individual susceptibility to obesity and thus help explain the recent large increases in obesity. This review discusses current advances in our understanding of how obesogens act to affect health and obesity susceptibility. Newly discovered obesogens and potential obesogens are discussed, together with future directions for research that may help to reduce the impact of these pervasive chemicals.

Obesity is a pandemic that has reached worldwide proportions, affecting essentially every country (1). The most dramatic increase in obesity incidence has occurred over the past 5 decades. Approximately 39.6% of US adults were characterized as obese in 2016, compared to 13.4% in 1980 (2). Even more alarming, the incidence of obesity in children has also been increasing, with 18.5% of US children being characterized as obese in 2016, compared to just 4% before 1980 (2). In some countries, the prevalence of childhood obesity exceeds that of adults (3). Obesity and high body mass index (BMI) are not just cosmetic concerns, but are also associated with comorbidities such as increased risk for heart disease, type 2 diabetes and other metabolic diseases, and cancers, and have contributed to approximately 4 million deaths worldwide between 1980 and 2015 (3). There is an urgent need to understand all the factors contributing to obesity to best implement effective prevention and treatment approaches that have so far proved elusive.

The Obesogen Hypothesis

The predominant medical explanation continues to be that obesity is the result of a simple imbalance between excessive calorie intake and insufficient energy expenditurethe energy balance or calories in, calories out model. However, recent studies have demonstrated that this simple paradigm cannot explain the increase in BMI seen in recent years. A study analyzing National Health and Nutrition Examination Survey (NHANES) data compared BMI between US adults in 1988 and 2006 and found a 2.3-kg/m² increase in adult BMI in 2006 compared with 1988, even at the same amount of caloric intake and energy expenditure (4). Moreover, the quality of carbohydrate calories consumed (high vs low glycemic load) appears to be more important than the total quantity of calories consumed (5, 6). Genetics is widely believed to be associated with obesity; however, known gene variants can explain only 2.7% of the individual variation in BMI (7). Therefore, the 2 most commonly given explanationsgenetics and energy balancecannot fully explain the substantial increases in obesity incidence observed worldwide.

Multiple environmental factors can affect obesity susceptibility (reviewed in 8, 9). These include the gut microbiome composition (10, 11), stress (12), and disrupted circadian rhythms (13), to name a few. Environmental stressors experienced during fetal development have significant impacts on obesity susceptibility later in life. For example, mothers who were in their first and second trimester of pregnancy during the Dutch Hunger Winter of 1944 to 1945 gave birth to children who were predisposed to obesity later in life compared to mothers not exposed to famine during pregnancy (14). Maternal smoking during pregnancy was also shown to lead to a predisposition toward obesity later in life for prenatally exposed children (reviewed in 15).

In 2003, Jerry Heindel put endocrine-disrupting chemicals (EDCs) and obesity on the same map for the first time (16). His idea followed from a proposal originally made by Paula Baillie-Hamilton that increased chemical usage since World War II was responsible for the rapid increase in obesity over the same time period (17). Although it was not justifiable to link increased chemical use, per se, to obesity, Heindels proposal that EDCs might be influencing obesity was reasonable because nearly every aspect of the control of appetite, satiety, metabolism, and fat storage is regulated by the endocrine system. Heindels proposal eventually ignited research in the area of obesity among researchers already working on EDCs (reviewed in 18).

The idea of EDCs as factors in obesity did not crystallize until it was recognized that certain EDCs could activate nuclear hormone receptors important for the development of white adipocytes, such as peroxisome proliferatoractivated receptor (PPAR) (19). Further support came from the findings that EDCs such as tributyltin (TBT) could lead to increased adipogenesis in cell culture models (20-22), bind to and activate PPAR and its heterodimeric partner, the 9-cis retinoid X receptor (RXR), in vitro (21, 22), and lead to increased adiposity in vivo (22). The identification of chemicals that activated PPAR to promote adipocyte differentiation and white adipose tissue (WAT) accumulation led to the coining of the term obesogen (23).

Obesogens were defined functionally as chemicals that lead to increased WAT accumulation, in vivo, after exposure. The environmental obesogen hypothesis holds that obesogen exposure is an under-recognized and understudied factor in the obesity pandemic (reviewed in 23, 24). Although the obesogen hypothesis was initially controversial, many chemicals known to be obesogenic in animal models are also associated with increased obesity prevalence, BMI, and body weight in humans (9). Research in this area has burgeoned and numerous recent reviews have summarized aspects of obesogen research (eg, 8, 18, 25, 26). It was subsequently recognized that obesogens may have more diverse effects on metabolism than just contributing to obesity; although, obesity may be a key contributor to such effects. These include type 2 diabetes, nonalcoholic fatty liver disease, and the central control of metabolism. Thus, it can be argued that most obesogens are a subset of a larger class of chemicals termed metabolism-disrupting chemicals, not all of which are obesogens (reviewed in 9, 27). Here we summarize what is known about the mechanisms underlying obesogen action, discuss newly identified obesogens and potentially obesogenic chemicals and propose important areas for future research.

Classic Effects and Mechanisms of Obesogens

Obesogens affect the differentiation of white adipocytes, in vitro, and the storage of fat, in vivo, in multiple model organisms (reviewed in 8, 9)(Fig. 1). We distinguish bona fide obesogens that induce increased WAT weight, in vivo, from potential obesogens that can induce adipogenesis, in vitro, but have not yet been demonstrated to induce WAT accumulation in vivo. Numerous potentially obesogenic compounds have been identified using in vitro assays that assess the ability of candidate chemicals to promote differentiation of established cell lines such as 3T3-L1 preadipocytes (28, 29) or primary mouse and human multipotent mesenchymal stromal stem cells (also known as mesenchymal stem cells or MSCs) into mature adipocytes (29, 30). Many chemicals that promote differentiation of white adipocytes in these assays activate PPAR and/or RXR (29, 30). This is not surprising because the PPAR:RXR heterodimer is considered to be the master regulator of adipogenesis (31).

Figure 1.

Diagram of known and proposed mechanisms and effects of obesogen exposure. Known mechanisms through which obesogens act are demonstrated by solid purple arrows. Proposed mechanisms are shown by arrows with a dashed black line. Plausible, but uninvestigated, interactions between obesogen actions are shown by arrows with a dashed gray line. Known mechanisms are described in green boxes, and the outcomes of obesogen exposures are shown in pale red boxes.

One of the most well-characterized obesogens is the organotin, TBT. Organotins are widely used in industry and to some extent in agriculture. Human exposure to organotins can occur via the diet such as seafood contaminated by TBT used in marine shipping applications (32), or as fungicides for paper mills and industrial water systems (reviewed in 33). Triphenyltin use as a fungicide and miticide on high-value food crops presents more opportunities for human exposure (34). TBT contaminates polyvinyl chloride plastics, and organotins are found in samples of house dust (35, 36).

TBT binds to and activates PPAR and RXR at environmentally relevant (nanomolar; nM) levels, promoting adipogenesis and lipid accumulation (21, 22, 37). Human and mouse MSCs and 3T3-L1 preadipocytes were induced to differentiate into white adipocytes via a PPAR-dependent pathway after exposure to nM levels of TBT (38, 39). Prenatal TBT exposure diverted bone marrowderived MSCs preferentially toward the adipose lineage and away from the bone lineage in exposed mice (39) and in mouse MSCs, in vitro (40).

Prenatal and/or perinatal exposure to TBT in mice led to increased body fat in the offspring (22, 41, 42). TBT also induced obesity in mice in both sexes when treated at any age (43-46). Similar effects were observed in rats (46), goldfish (47), and zebrafish (48, 49). Therefore, the obesogenic effects of TBT exposure, developmentally and in adulthood, are well supported in the literature across model systems.

Epidemiological studies of TBT exposures and effects are scant. A longitudinal Finnish cohort study positively associated placental TBT levels with infant weight gain, an established risk factor for adult obesity (50). A recent analysis of NHANES data revealed a strong link between elevated urinary total tin levels and diabetes (51). Human exposure to tin is ubiquitous (52), and it was just shown that plastic specimen containers strongly bind organotins (particularly TBT), sharply impairing recovery (53). Therefore, previous studies of organotin levels in human specimens (eg, 54) probably substantially underestimated TBT levels because of their use of plastic containers during processing and analysis.

A recent clinical study that strongly supports the obesogen hypothesis found that people with the highest blood levels of perfluorinated chemicals had lower resting metabolic rates and regained weight faster after dieting than those with the lowest levels (55). In agreement with predictions from rodent studies (39, 40), the same group showed that humans with the highest blood levels of perfluoroalkyl compounds had lower bone mineral density at baseline and lost bone mineral density faster in a weight-loss trial (56).

Similar obesogenic effects have been observed with other environmental chemicals, such as phthalates, persistent organic pollutants, and components of plastics and epoxy resins. The phthalate MEHP (mono-2-ethylhexyl phthalate) induced adipogenesis in 3T3-L1 preadipocytes via activation of PPAR (57). DEHP (di-2-ethylhexyl phthalate) induced expression of adipogenic genes in vivo and an obesity phenotype in mice following perinatal exposure (58). Prenatal exposure to bisphenol A (BPA) has been linked to various adverse health effects including reproductive and behavioral issues (59), fat gain in mice and rats (60, 61) and other metabolic outcomes such as type 2 diabetes (62, 63). Some data support the possibility that BPA influences adipogenesis as a PPAR agonist, although BPA is a relatively weak activator of PPAR in vitro (64-66). Others have proposed that BPA may induce its obesogenic effects indirectly via its ability to bind to estrogen receptors and interfere with estrogen signaling (67).

Effects of obesogens acting through other nuclear receptors such as the glucocorticoid receptor, estrogen receptors, and androgen receptors have been reported and discussed in detail elsewhere (reviewed in 9, 26). More recently, it has been observed that chronic 52-week exposure of male mice to a mixture of 6 pesticides commonly used in France, at doses equivalent to the tolerable daily intake of each pesticide, led to increased body weight, WAT weight, and glucose intolerance (68). In contrast, similarly exposed female mice exhibited elevated fasting glucose, increased ratio of reduced to oxidized glutathione in the liver, and perturbed levels of microbiome-related urinary metabolites. Loss of the xenobiotic receptor constitutive androstane receptor (CAR) prevented body weight gain and changes in glucose metabolism in male mice, whereas females exhibited increased toxicity, higher body weights, and elevated mortality rates in the absence of CAR (68).

Many obesogens that have been identified using in vivo studies act through nuclear hormone receptordependent mechanisms, as do many of those detected using in vitro adipogenesis assays (reviewed in 69). In addition, interaction between nuclear receptors and cross-talk between signaling pathways has been reported (70). However, some of the effects observed in vitro (29) and in vivo, especially transgenerational effects, (25) have not been linked directly to the activation of particular nuclear hormone receptors. Therefore, the mechanisms through which these compounds act appear to be more complex.

Mechanisms of Obesogen Action: Beyond Peroxisome ProliferatorActivated Receptor

Because many known obesogens activate PPAR and induce adipogenesis, PPAR activation is widely believed to be a major mechanism through which obesogens can contribute to obesity. PPAR continues to be the receptor most commonly targeted in screening assays for obesogens (28, 71, 72). However, recent studies have demonstrated alternative and novel mechanisms of obesogen action. These include activation of RXR to induce adipocyte lineage commitment and impair adipocyte health (73, 74), activation of multiple other nuclear receptors, induction of epigenetic modifications in fat tissue (75), alteration of chromatin accessibility or architecture (41, 76), and induction of gut microbiome dysbiosis (77-79). Thus, obesogens have a broad and diverse spectrum of actions. This section discusses some PPAR-independent mechanisms of obesogens action. A summary of the known and proposed mechanisms through which obesogens can act, together with their possible effects, is illustrated in Fig. 1.

Adipocyte commitment

TBT was shown to bind to and activate RXR, which is an obligate heterodimeric partner for many nuclear receptors, including PPAR (80). TBT, as well as selective RXR activators (rexinoids), could commit female mouse MSCs to the adipocyte lineage by activating RXR, whereas selective pharmacological activation of PPAR could not (74). It was proposed that RXR activation in MSCs inhibits the expression of enhancer of zeste 2, the catalytic subunit of polycomb repressor complex 2, which deposits the repressive histone 3 lysine 27 trimethylation mark (H3K27^me3) on chromatin. Loss of H3K27^me3 in TBT-treated MSCs near to genes important for adipogenic commitment led to increased expression of these genes, which largely explains the increased commitment of these MSCs toward the adipose lineage (74). These results also demonstrated that TBT activation of RXR can induce epigenomic modifications, revealing a new mode of action for this well-known toxicant.

Adipocyte health

Adipocytes have important functions in the maintenance of metabolic health, including glucose and triglyceride uptake from the bloodstream in response to insulin. Disruption of adipocyte function contributes to insulin resistance and type 2 diabetes. Regnier and colleagues first showed that adipocytes induced to differentiate from 3T3-L1 cells by TBT were functionally distinct from adipocytes induced by the PPAR activator, troglitazone (81). It is generally accepted that selective PPAR activators, such as troglitazone and rosiglitazone, promote the development of healthy white adipocytes in vitro and in vivo (82, 83). Healthy white adipocytes are characterized by sensitivity to insulin; production of insulin sensitizing, anti-inflammatory adipokines such as adiponectin, apelin, and fibroblast growth factor 21; normoxia; and low to no expression of inflammatory or fibrotic marker genes. Healthy white adipocytes have the ability to undergo conversion to beige adipocytes when treated with thyroid hormone, cold exposure, or 3-adrenergic receptor agonists. These thermogenic adipocytes express genes such as Ucp1 to uncouple oxidative phosphorylation from adenosine 5-triphosphate production, leading to the production of heat (73). Activation of RXR by TBT and other rexinoids not only led to increased adipogenic commitment, but the adipocytes treated with TBT or rexinoids during differentiation from MSCs were dysfunctional (73). These adipocytes displayed impaired glucose uptake and insulin signaling, increased expression of inflammatory and fibrotic markers, decreased respiratory function, and reduced expression of beige/brown adipocyte marker genes such as Ucp1, Elovl3, Cidea, and PPAR when stimulated with thyroid hormone (73). TBT also failed to induce marker genes characteristic of thermogenic beige/brite adipocytes in a transcriptomal analysis of bone marrowderived MSCs (84). There is also some evidence that other obesogens can impair thermogenesis in vivo. For example, perinatal exposure to the obesogen DDT led to impaired thermogenesis in the brown adipose tissue, resulting in lower core body temperature and increased susceptibility to high-fat dietinduced insulin resistance in adult female rats (85). Together, these results revealed a new mechanism for obesogen action independent of PPAR activation. They also demonstrated that some obesogen-induced adipocytes may be dysfunctional, compounding the negative effects of promoting increased accumulation of white fat. Additional in vivo studies will be needed to ascertain the degree to which obesogen exposure alters adipocyte health and how this influences the predisposition to obesity-related diseases.

Gut microbiome dysbiosis

A relatively unexplored mechanism through which obesogen exposure might predispose exposed individuals to obesity is via alterations to the gut microbiome. It is well established that obesity is associated with composition of the gut microbiome (86, 87). Transplant of the gut microbiome from obese individuals can induce obesity in germ-free mice (11). In contrast, transplant of the microbiome from a lean individual promoted a lean phenotype in similar experiments (11). Transplant of the microbiome from lean donors improved metabolic end points in recipients with metabolic syndrome (11, 88).

Many xenobiotics, including known obesogens, induced changes to gut microbiome composition (77, 89-91). For example, BPA exposure led to increased prevalence of Proteobacteria and Helicobacteraceae together with reduced Clostridia in the gut microbiota of exposed mice, but the study did not investigate whether the altered microbiome was directly associated with any metabolic end points (89). TBT exposure was associated with changes to the gut microbiome in mice, including increased prevalence of Proteobacteria and Helicobaacteraceae, and decreased prevalence of Clostridia, Bifidobacteriaceae, and lactobicillaceae (77). Exposure to triphenyl phosphate increased abundance of Erysipelotrichia and Bacilli and also decreased the prevalence of Clostridia (79). Gut microbial dysbiosis in mice following exposure to triphenyl phosphate (79) or TBT (77) was associated with increased fat accumulation or altered lipid metabolism. However, these studies did not distinguish whether observed differences in the microbiome were the result of metabolic differences in the mice or if the altered microbiome induced the metabolic changes. Monitoring the microbiomes of the dams before and after exposure could help in differentiating between these possibilities, as would conducting a microbiome transplant into germ-free mice. Also, microbial metabolites have been identified as agonistic ligands for the aryl hydrocarbon receptor (AhR) (reviewed in 92). Promoting AhR activity inhibited adipogenesis (93), obesity, and fatty liver both in male and female mice (94, 95) and protected from the effects of a high-fat diet (96). Inhibiting the expression or action of AhR promoted obesity and fatty liver (reviewed in 97).

The Western dietary pattern is strongly associated with obesity, and several recent studies have demonstrated that components of the Western diet (eg, ultra-processed food, food additives, artificial sweeteners) can disrupt the gut microbiome (98). Two common dietary emulsifiers, carboxylmethylcellulose and P-80, were recently shown to induce intestinal inflammation and disrupt the gut microbiome, leading to metabolic syndrome in mice together with increased body weight and WAT depot weight (99). (See also Surfactants and Food additives sections for further discussion on similar emulsifiers and food additives.) Taken together, these results suggest that disrupting the composition of the gut microbiome could be a new mechanism through which obesogen exposure can promote obesity. They also reveal common food additives as a new class of candidate obesogens that require further investigation.

Possible Mechanisms Underlying Transgenerational Effects of Obesogen Exposure

An intriguing result is that the effects of early-life obesogen exposure can be transmitted to future generations. When pregnant F0 mouse dams were treated with TBT, F1 animals were exposed as embryos, and F2 were exposed as germ cells within F1. F3 and subsequent generations were not exposed; effects in these generations are considered to be transgenerational and permanent (100, 101). It was shown that the effects of TBT treatment on obesity were transgenerational and could be detected in the F1, F2, F3, and F4 descendants of F0 mice exposed during pregnancy (42) or during pregnancy and lactation (41). Interestingly, transgenerational obesity was not observed in similar experiments using the strong PPAR activator rosiglitazone; therefore, pathways in addition to PPAR may be required to produce the transgenerational phenotype (42). Alternatively, it might be possible that the unhealthy adipocytes produced by TBT exposure are responsible for the transgenerational phenotypes in adipose tissue; this is a ripe area for future study. It was proposed that obesogen exposure can permanently reprogram MSCs to favor the adipose lineage (39). Gene expression in MSCs taken from F1 to F3 generation mice after F0 exposure throughout pregnancy was also biased toward the adipogenic lineage (42). It was suggested that TBT exposure promoted epigenomic changes favoring the development of obesity (41); thus, this may be an example of a maternal programming event leading to a life-long phenotype.

In addition to TBT, heritable effects of several environmental chemicals on obesity have been demonstrated, albeit at relatively high doses. Plastic components such as BPA, diethylhexyl and dibutyl phthalates (58), the pesticide methoxychlor (102), a mixed hydrocarbon mixture (jet fuel JP-8) (103), and the once widely used pesticide, DDT (104) all induced transgenerational obesity in rats, observed in the F3 and/or F4 offspring of following ancestral prenatal or perinatal obesogen exposure to the F0 dams (58, 101-104). The mechanisms underlying these transgenerational effects remain unclear. Some proposed mechanisms for transgenerational effects of obesogen exposure are discussed in this section and illustrated in Fig. 1. Transgenerational effects of obesogen exposure are particularly concerning because current risk assessment paradigms do not consider this generational toxicology (105).

Epigenetic modifications

Epigenetic modifications to the genome are one mechanism through which environmental factors, such as chemical exposure, can alter gene expression and lead to adverse outcomes. Many epigenetic modifications have been observed after obesogen exposure. For example, the obesogen TBT induced global changes to DNA methylation (106) and to histone methylation (74) in vitro. Many environmental chemicals, including known obesogens, led to epigenetic modifications in vivo and altered epigenetic signatures and obesity phenotypes in unexposed generations (58, 75, 103, 104). It has been suggested that such epimutations can be inherited (58, 104, 107) and that chemical exposures during fetal development can induce epigenetic changes in the germline leading to observed phenotypes in subsequent generations (108).

Some argue that altered DNA methylation itself may be transmitted across generations (109, 110), but others contest these results and hold that DNA methylation is not readily heritable (111, 112). This is because the zygote undergoes genome-wide erasure of epigenetic marks shortly after fertilization, and the developing germ cells experience an additional wave of global demethylation as they mature (113-115). These stages of global demethylation both in the zygote genome and in the developing germline would likely prevent inheritance of most altered DNA methylation marks. There is some discussion about some DNA methylation escaping reprogramming in an imprinting-like mechanism; however, the presence of DNA methylation at particular sites has not been demonstrated across generations. Indeed, the opposite has been shown; there is little, if any consistency in DNA methylation across generations and among tissues in transgenerational experiments (75, 105, 116, 117). The consensus from a variety of exposures is that altered DNA methylation can be detected in the F3 generation and beyond in transgenerational experiments, but a plausible mechanism for how these changes are transmitted across generations is lacking.

In addition to DNA methylation, other types of epigenetic changes have been observed following ancestral chemical exposure. Histone modifications are capable of inducing changes to chromatin packaging and therefore, DNA accessibility and gene expression. In the male germline, a majority of histones are removed during spermatogenesis. However, 5% to 15% of histones are retained in mammalian sperm. Analysis of the sperm of F3 rats ancestrally exposed to the pesticide DDT or the fungicide vinclozolin revealed additional differential histone retention sites for histone H3 compared to the sperm of control lineage F3 animals (118). Histone retention in the sperm can be affected by environmental toxicant exposure and might be transmitted to unexposed generations. However, the underlying mechanisms and the importance of these retention sites on the development of disease phenotypes require further investigation. Noncoding RNA (ncRNA) expression is another type of epigenetic change that has been observed in transgenerational experiments. More than 200 differentially expressed small noncoding RNAs (sncRNAs) were identified in the sperm between F3 rats ancestrally exposed to vinclozolin compared with controls (119). Some of these dysregulated sncRNAs correlated with messenger RNA profiles observed in diseased tissues in animals ancestrally exposed to vinclozolin. Although differences both in ncRNA and differential histone retention sites have been observed in transgenerational experiments after exposure to environmental toxicants, these changes have not been linked mechanistically to transgenerational obesity.

Chromatin accessibility

One potential explanation for how epigenomic changes are observed in unexposed generations was recently proposed (41, 76). In this model, TBT exposure induced global changes to chromatin organization that resulted in changes in DNA methylation. Epigenetic analysis of the fat tissue of obese F4 male mice ancestrally exposed to the obesogen TBT revealed more than 10000 regions where methylation was altered in 4 out of 4 animals from the TBT group vs controls. However, none of these regions was closely associated with the promoters of genes whose expression was altered in these animals. When methylation was analyzed in a different way, assessing larger blocks of differentially methylated regions in genomic DNA with the same direction of methylation, the result was different (41). These regions were called isodirectionally differentially methylated blocks, or isoDMBs. Hypomethylated isoDMBs were associated with overexpression of metabolism related genes, such as leptin, in fat tissue of F4 mice ancestrally exposed to TBT (41). Investigation into chromatin accessibility of the sperm of F3 or F4 mice revealed that the hypomethylated isoDMBs in the WAT coincided with decreased chromatin accessibility in the same regions of the sperm (41). It was proposed that TBT exposure altered chromatin architecture, which resulted in decreased chromatin accessibility of regions where genes important for metabolism were located, producing a thrifty phenotype (41).

A deeper analysis of liver, MSCs, and WAT from the same experiment revealed that the transcriptomes of tissues of mice ancestrally exposed to TBT showed a bias in the expression of genes related to chromatin organization, chromosome organization, and metabolic processes compared with the control group and that these differences spanned generations (F3, F4), tissue types (liver, MSCs, WAT), and ontogeny (mesoderm vs endoderm) (76). The authors inferred that TBT exposure disrupted chromatin and chromosome organization and that this disrupted structure was able to self-reconstruct in subsequent generations, much as the normal structure is able to do in controls (76). In this model, the disrupted chromatin structure leads to alterations in DNA methylation, histone retention, and the expression of messenger RNAs as well as ncRNAs rather than these epigenomic alterations being inherited directly (76). However, the molecular mechanisms underlying this disrupted structure and how it might self-reconstruct in subsequent generations remain unknown at present.

Newly Discovered Obesogens (New Threats)

Obesogens have been extensively reviewed in recent years and several publications report and categorize known obesogens (reviewed in 8, 26, 69). Additional chemicals are being identified that may act as obesogens in vitro and in vivo. This section summarizes recent findings concerning new potential obesogens and how their action (when known) compares with that of model obesogens, such as TBT.

Dibutyltin

Dibutyltin (DBT) is the major breakdown product of TBT in vivo and is more prevalent in the environment than TBT because of its presence at substantial concentrations (up to 3% w/w) in polyvinyl chloride (PVC) plastics (120). DBT has been demonstrated to leach into drinking water from PVC pipes and, therefore, may produce a hazard to humans (121). DBT activated the same receptors as does TBT and induced 3T3-L1 preadipocytes (122) and human and mouse MSCs to differentiate into adipocytes (123). In addition, perinatal exposure to DBT led to increased WAT weight in mice comparable to that of the model obesogen TBT, although a higher dose of DBT was required to achieve the same effect as TBT (123). Surprisingly, offspring of DBT-exposed dams demonstrated insulin resistance, a phenotype not observed with TBT exposure (123). This raises the possibility that DBT may engage additional cellular mechanisms to those through which TBT acts. The effects of DBT on other phenotypes elicited by TBT, such as reprogramming stem cell fate, fatty liver, impairing thermogenesis, and transgenerational transmission have not yet been investigated.

Bisphenol A analogues

Considerable evidence on the adverse health effects of BPA exposure has led to efforts to produce BPA-free plastics. However, related chemicals such as bisphenol S (BPS) and bisphenol F (BPF), are often used to replace BPA in these new plastics as companies strive to develop BPA-free products but retain current manufacturing processes (124). The toxicity of these BPA analogues is less well understood. Some studies have demonstrated that these bisphenols also have endocrine disrupting properties similar to BPA (reviewed in 125). Interestingly, BPS and halogenated BPA analogues demonstrated higher activation of PPAR and potency in inducing adipogenesis than did BPA (64, 65). A recent study revealed that perinatal exposure to BPS elicited obesity in mice (126). Although exposure levels of BPA have been significantly associated with obesity incidence, levels for BPS and BPF were not linked with obesity in a cross-sectional study of adults after adjusting for lifestyle and socioeconomic factors (124). Interestingly, a newer longitudinal birth cohort study revealed that BPS and BPF were significantly associated with obesity in children (age 6-19), whereas BPA and total bisphenol levels were not significantly associated (127). These results suggest substituting other bisphenols for BPA may not be an effective strategy for mitigating the hazards of BPA to humans.

Acrylamide

Acrylamide is a chemical widely used in the manufacture of paper, dye, and other industrial products. It can also be formed as an unintentional byproduct of cooking carbohydrate-containing foods at high temperatures by frying, baking, or roasting, which is probably the main source of human exposure (128). A recent study reported that acrylamide exposure induced fat accumulation in male mice when fed a high-fat diet (129). Although acrylamide apparently increased PPAR expression, it was not identified as a PPAR activator. Instead, it was that acrylamide acted through the mitogen-activated protein kinasd and AMPK-ACC (adenosine 5-monophosphateactivated protein kinaseacetyl-CoA carboxylase) pathways (129). Studies in 2 different longitudinal birth cohort studies from (France) (130) and Norway (131) demonstrated that children prenatally exposed to higher levels of acrylamide were more likely to be born small for gestational age and obese at age 3 years. Hemoglobin adducts of acrylamide (HbAA) and glycidamide (HbGA) were proposed as biomarkers of acrylamide exposure in humans. One analysis of NHANES data (2003-2006) demonstrated a positive association between HbGA levels and obesity, but a negative association between HbAA and obesity (132). In contrast, another analysis of NHANES data (2003-2004) found a negative association with obesity for HbAA and no association with HbGA (133). Clearly more data are required to establish whether acrylamide exposure is linked with obesity, but the ubiquitous exposure of the population to acrylamide from baked and fried foods indicates that such data will be very important.

Surfactants

Dioctyl sodium sulfosuccinate (DOSS) is commonly used as a dietary emulsifier and as a major component of an over-the-counter and commonly recommended stool softener (Colace/Docusate). DOSS was also a principal component in the COREXIT (Corexit Environmental Solutions LLC, Nalco Holding Company) dispersants that were used in the cleanup of the Deepwater Horizon oil spill in 2010 (134). DOSS activated PPAR and induced adipogenesis in vitro (135). A recent in vivo mouse study showed that perinatal exposure of pregnant mouse dams to DOSS led to obesity in their offspring (136). A second commonly used surfactant (and component of COREXIT), Span-80 (sorbitan monooleate, Croda International PLC.), activated RXR and induced 3T3-L1 preadipocytes to differentiate into adipocytes (137). When 3T3-L1 cells were treated with a combination of Span-80 and DOSS, adipogenic induction was greater than with either chemical individually (137). These results establish surfactants as an unexplored category of obesogens requiring further investigation. Because both of these chemicals are commonly used as food additives, it will also be interesting to test whether they affect the gut microbiome to induce obesity phenotypes in vivo, as other dietary emulsifiers have been shown to do (99).

Food additives

Increasing evidence has emerged linking components of the Western dietary pattern (eg, ultra-processed, food additives) to obesity. Notably, commonly used food additives have been shown to have obesogenic potential. The dietary emulsifiers carboxymethylcelluclose and P-80 (see Gut Microbiome Dysbiosis section) as well as DOSS and Span-80 (see Surfactants section) induced adipogenesis in vitro and/or in vivo. Other food additives have also been shown to have obesogenic potential. The widely used food preservative 3-tert-butyl-4-hydroxyanisole (3-BHA) induced adipocyte differentiation in 3T3-L1 preadipocytes (138), and 3-BHA exposure increased adiposity and lipid plasma levels in exposed mice (139). The flavor enhancer MSG (monosodium glutamate) was long ago shown to be an obesogen in vivo (140). Two studies showed that MSG may induce its adipogenic effects by impairing secretion of glucagon-like peptide-1, an important hormone regulating appetite and satiety (141) and/or by antagonizing androgen receptor action (142). Taken together, these studies provide insight into why the consumption of highly processed food leads to greater weight gain than consumption of the same number of calories from fresh foods (5, 6). This is a ripe area for future investigation.

Pesticides

Many pesticides or their major breakdown products have been linked to obesity in animals and in humans (reviewed in 143). One example is DDT in rats (104) and humans (144) and its major breakdown product DDE in humans (145). Although DDT was banned under the Stockholm Convention, it persists in the environment and continues to be used in some countries (particularly in Africa). Methoxychlor induced obesity in rats (102), and other pesticides have been identified as candidate obesogens. The widely used, neonicotinoid insecticide, imidacloprid, was recently shown to induce 3T3-L1 preadipocytes to differentiate into adipocytes (as measured by lipid accumulation and marker gene expression) (146) and to promote high-fat dietinduced obesity in mice (147). Glyphosate, the most highly used herbicide worldwide, induced obesity in F2 and F3 offspring of F0 female rats exposed during gestation (148). The herbicide quizalofop-p-ethyl induced adipogenesis in 3T3-L1 preadipocytes (149). A variety of other agrochemicals induced adipogenesis in 3T3-L1 preadipocytes and in mouse and human MSCs (29, 30). Although the potential of many of these agrochemicals to promote obesity in vivo has not yet been explored, the intensive use of such chemicals and the widespread human exposure via consumption of foods indicates that such studies will be important to understand the contributions of agrochemical exposure to obesity.

Future Directions

Although the environmental obesogen field is just 15 years old, it is becoming clear that chemical exposures may be important contributors to the obesity pandemic. Many advances have been made into potential mechanisms underlying obesogen action and how obesogen exposure may predispose humans and animals to obesity (Fig. 1). However, we have only just scratched the surface and need to learn much more about the number of obesogens that exist, how they act, and how we can best protect ourselves and future generations from their harmful impacts. A combination of mechanistic studies in cell and animal models together with longitudinal epidemiological and biomonitoring studies in humans will be required for a full assessment of the risks and costs of these exposures to public health. Early estimates suggest that these costs may be substantial (150, 151).

Many of the recent discoveries regarding the mechanisms of obesogen action are still in the early stages and require more research to determine their significance in obesity susceptibility. We currently know very little about how obesogen exposure can interact with diet to promote obesity. We know that obesogens can affect composition of the microbiome and that microbiome composition itself can cause obesity. Yet we know little about how obesogen-elicited changes in the microbiome can contribute to obesity or whether observed changes in microbiome composition are the cause or consequence of obesity and obesogen-induced metabolic abnormalities.

Sexually dimorphic effects of obesogens are quite common. The first chemical to be reported as an obesogen in vivo was the synthetic estrogen diethylstilbestrol, which elicited obesity after perinatal exposure only in adult female mice (152). Such effects might be mediated via the estrogen receptors, but this remains to be demonstrated. TBT elicited increased fat mass in both sexes of the F1 generation, but obesity was found only in males of the F2 to F4 generations and it was not possible to link these effects with sex steroid receptors (41, 42, 123). Many other examples of sexually dimorphic effects of obesogen exposure exist in animal models (reviewed in 9). The incidence of obesity is increasing in both sexes in human populations; however, the effect is most striking in female patients, particularly in the United States (2). We currently know very little about the etiology of these sexual dimorphisms beyond some indications that effects of environmental estrogens, such as BPA, may be expected to be more pronounced in girls and women. Because most obesogens do not act via the estrogen receptors, it will be important to understand which pathways mediate obesogen action to formulate appropriate strategies for intervention and prevention.

It will be important to understand the effects of mixtures on obesity and whether combined exposure of obesogens will interact in a positive or synergistic manner, or if they will instead cancel each others effects. Many obesogens appear to induce a variety of effects and, therefore, may be acting through multiple mechanisms. TBT, for example, activates PPAR and RXR, but also induces epigenetic modifications and changes to chromatin architecture (41, 76). However, it is not known how these changes in chromatin architecture can be transmitted to future generations. Multiple chemicals have been demonstrated to elicit transgenerational effects on obesity, but we know virtually nothing about how these effects are carried across generations (reviewed in 69, 105). Nuclear receptor activation can lead to epigenetic alterations (153, 154), but this connection has not yet been characterized as part of the mechanism of action of obesogens. Many studies have established that the microbiome can be linked to epigenetic changes (155, 156), but it remains to be shown whether the effects of obesogen exposure on the microbiome and the epigenome are causally related. It is also possible that the impact of obesogens on the obesity pandemic could be the result of exposure to a combination of obesogens, each of which may act through a different pathway, rather than through a single pathway. Many studies have found that chemical mixtures can induce higher receptor activation or stronger phenotypes (157-160).

Although we have learned quite a bit about the number and nature of obesogens and have gleaned clues to how some act, we know relatively little about the full spectrum of obesogens and their mechanisms of action. Understanding how obesogens act will pave the way for identifying new obesogens that may act through similar mechanisms. What is needed for EDCs in general, and obesogens in particular, are better screening systems that can identify likely candidates for in-depth screening. ToxCast and Tox21 have been touted as the future of such screening studies but they may not be adequate for the task (29). It will be important to establish standardized, internationally harmonized screening methods that are reliable, robust, and perform reproducibly across laboratories. The European Union under its Horizon 2020 program has funded 8 international consortia to develop such screening assays to identify EDCs. Three of these consortia are focused on developing methods to identify metabolism-disrupting chemicals. Such efforts will lay the foundation for international efforts to identify potential EDCs and obesogens. Because some of these chemicals may have multigenerational or transgenerational effects, their identification should be an urgent public health goal.

Lastly, one may wonder to what extent the effects of obesogen exposure can be treated and whether it is possible to avoid exposure to such chemicals. One unfortunate consequence of living in an industrialized society is exposure to a plethora of synthetic chemicals. Despite substantial evidence for the widespread effects of EDCs on laboratory animals, wildlife, and humans, changing public policy to limit exposures has proven to be difficult. No doubt it will be equally difficult to implement public policies designed to reduce exposure to obesogens. It seems to be much more likely that effective changes in public policy will be best implemented at the level of local cities and school boards where the influence of concerned citizens equals or exceeds that of industry lobbyists, compared with the state and federal levels where the opposite prevails. Certainly, this has proven to be the case in Southern California, where a number of cities and school districts have implemented policies to greatly reduce exposure to toxic chemicals, including EDCs. For the foreseeable future, the most effective approach will probably be personal action to reduce or eliminate our exposures. We recommend implementing a personal precautionary principle whereby we take steps to eliminate EDCs and obesogens in our own diets, personal care products, and lifestyles to the extent possible. The rise in availability of fresh organically grown foods, personal care products lacking EDCs, and cleaning products lacking toxic ingredients, along with an increase in movements to reduce the use of single-use plastics, for example, is testimony to the powerful effects that our buying choices in the marketplace have on corporate behavior.

Abbreviations

AhR
aryl hydrocarbon receptor
BMI
BPA
BPF
BPS
CAR
constitutive androstane receptor
DBT
DDT
dichlorodiphenyltrichloroethane
DEHP
di-2-ethylhexyl phthalate
DOSS
EDCs
endocrine-disrupting chemicals
HbAA
hemoglobin adducts of acrylamide
HbGA
hemoglobin adducts of glycidamide
isoDMBs
isodirectionally differentially methylated blocks
MEHP
mono-2-ethylhexyl phthalate
MSCs
NHANES
National Health and Nutrition Examination Survey
PPAR
peroxisome proliferatoractivated receptor
PVC
RXR
TBT
WAT

Acknowledgment

Financial Support:This work is supported by the National Institutes of Health (Grant ES023316 to B.B.).

Additional Information

Disclosure Summary:B.B. is a named inventor on US patents related to PPAR and other nuclear receptors, some of which have been licensed to for-profit entities. None of these present any conflict of interest. R.J.E. has nothing to disclose.

References

Hales

Carroll

Fryar

Ogden

Prevalence of obesity among adults and youth: United States, 2015-2016

NCHS Data Brief

2017

;(288):

GBD 2015 Obesity Collaborators;

Afshin

Forouzanfar

Reitsma

, et al.

Health effects of overweight and obesity in 195 countries over 25 years

N Engl J Med

2017

;

377

(

Brown

Sharma

Ardern

Mirdamadi

Kuk

Secular differences in the association between caloric intake, macronutrient intake, and physical activity with obesity

Obes Res Clin Pract.

2016

;

(

243

255

Ludwig

Ebbeling

The carbohydrate-insulin model of obesity: beyond calories in, calories out

JAMA Intern Med.

2018

;

178

(

1098

1103

Ludwig

Tappy

Brand-Miller

Dietary carbohydrates: role of quality and quantity in chronic disease

BMJ.

2018

;

361

k2340

Locke

Kahali

Berndt

, et al.;

LifeLines Cohort Study; ADIPOGen Consortium; AGEN-BMI Working Group; CARDIOGRAMplusC4D Consortium; CKDGen Consortium; GLGC; ICBP; MAGIC Investigators; MuTHER Consortium; MIGen Consortium; PAGE Consortium; ReproGen Consortium; GENIE Consortium; International Endogene Consortium

Genetic studies of body mass index yield new insights for obesity biology

Nature.

2015

;

518

(

7538

197

206

Heindel

Blumberg

Environmental obesogens: mechanisms and controversies

Annu Rev Pharmacol Toxicol.

2019

;

106

Heindel

Blumberg

Cave

, et al.

Metabolism disrupting chemicals and metabolic disorders

Reprod Toxicol.

2017

;

10.

Tilg

Kaser

Gut microbiome, obesity, and metabolic dysfunction

J Clin Invest.

2011

;

121

(

2126

2132

11.

Turnbaugh

Ley

Mahowald

Magrini

Mardis

Gordon

An obesity-associated gut microbiome with increased capacity for energy harvest

Nature.

2006

;

444

(

7122

1027

1031

12.

Torres

Nowson

Relationship between stress, eating behavior, and obesity

Nutrition.

2007

;

(

11-12

887

894

13.

Froy

Metabolism and circadian rhythmsimplications for obesity

Endocr Rev.

2010

;

(

14.

Ravelli

Stein

Susser

Obesity in young men after famine exposure in utero and early infancy

N Engl J Med.

1976

;

295

(

349

353

15.

Shan

Rehm

Rogers

, et al.

Trends in dietary carbohydrate, protein, and fat intake and diet quality among US adults, 1999-2016

JAMA.

2019

;

322

(

1178

1187

16.

Heindel

Endocrine disruptors and the obesity epidemic

Toxicol Sci.

2003

;

(

247

249

17.

Baillie-Hamilton

Chemical toxins: a hypothesis to explain the global obesity epidemic

J Altern Complement Med.

2002

;

(

185

192

18.

Heindel

History of the obesogen field: looking back to look forward

Front Endocrinol (Lausanne).

2019

;

19.

Hurst

Waxman

Activation of PPARalpha and PPARgamma by environmental phthalate monoesters

Toxicol Sci.

2003

;

(

297

308

20.

Inadera

Shimomura

Environmental chemical tributyltin augments adipocyte differentiation

Toxicol Lett.

2005

;

159

(

226

234

21.

Kanayama

Kobayashi

Mamiya

Nakanishi

Nishikawa

Organotin compounds promote adipocyte differentiation as agonists of the peroxisome proliferator-activated receptor gamma/retinoid X receptor pathway

Mol Pharmacol.

2005

;

(

766

774

22.

Grn

Watanabe

Zamanian

, et al.

Endocrine-disrupting organotin compounds are potent inducers of adipogenesis in vertebrates

Mol Endocrinol.

2006

;

(

2141

2155

23.

Grn

Blumberg

Environmental obesogens: organotins and endocrine disruption via nuclear receptor signaling

Endocrinology.

2006

;

147

(

6 Suppl

S50

S55

24.

Janesick

Blumberg

Endocrine disrupting chemicals and the developmental programming of adipogenesis and obesity

Birth Defects Res C Embryo Today.

2011

;

(

25.

Chamorro-Garcia

Blumberg

Current research approaches and challenges in the obesogen field

Front Endocrinol (Lausanne).

2019

;

167

26.

Darbre

Endocrine disruptors and obesity

Curr Obes Rep.

2017

;

(

27.

Papalou

Kandaraki

Papadakis

Diamanti-Kandarakis

Endocrine disrupting chemicals: an occult mediator of metabolic disease

Front Endocrinol (Lausanne).

2019

;

112

28.

Pereira-Fernandes

Demaegdt

Vandermeiren

, et al.

Evaluation of a screening system for obesogenic compounds: screening of endocrine disrupting compounds and evaluation of the PPAR dependency of the effect

PloS One.

2013

;

(

e77481

29.

Janesick

Dimastrogiovanni

Vanek

, et al.

On the utility of ToxCast and ToxPi as methods for identifying new obesogens

Environ Health Perspect.

2016

;

124

(

1214

1226

30.

Foley

Doheny

Black

, et al.

Editors highlight: screening toxcast prioritized chemicals for PPARG function in a human adipose-derived stem cell model of adipogenesis

Toxicol Sci.

2017

;

155

(

100

31.

Tontonoz

Spiegelman

Fat and beyond: the diverse biology of PPARgamma

Annu Rev Biochem.

2008

;

289

312

32.

Mattos

Stotz

Romero

Bravo

Fillmann

Castro

Butyltin contamination in Northern Chilean coast: is there a potential risk for consumers?

Sci Total Environ.

2017

;

595

209

217

33.

Lagadic

Katsiadaki

Biever

, et al.

Tributyltin: advancing the science on assessing endocrine disruption with an unconventional endocrine-disrupting compound

Rev Environ Contam Toxicol.

2018

;

245

127

34.

Golub

Doherty

Triphenyltin as a potential human endocrine disruptor

J Toxicol Environ Health B Crit Rev.

2004

;

(

281

295

35.

Fromme

Mattulat

Lahrz

Rden

Occurrence of organotin compounds in house dust in Berlin (Germany)

Chemosphere.

2005

;

(

1377

1383

36.

Kannan

Takahashi

Fujiwara

Mizukawa

Tanabe

Organotin compounds, including butyltins and octyltins, in house dust from Albany, New York, USA

Arch Environ Contam Toxicol.

2010

;

(

901

907

37.

le Maire

Grimaldi

Roecklin

, et al.

Activation of RXR-PPAR heterodimers by organotin environmental endocrine disruptors

EMBO Rep.

2009

;

(

367

373

38.

Ycaza

Blumberg

The environmental obesogen tributyltin chloride acts via peroxisome proliferator activated receptor gamma to induce adipogenesis in murine 3T3-L1 preadipocytes

J Steroid Biochem Mol Biol.

2011

;

127

(

1-2

39.

Kirchner

Kieu

Chow

Casey

Blumberg

Prenatal exposure to the environmental obesogen tributyltin predisposes multipotent stem cells to become adipocytes

Mol Endocrinol.

2010

;

(

526

539

40.

Yanik

Baker

Mann

Schlezinger

Organotins are potent activators of PPAR and adipocyte differentiation in bone marrow multipotent mesenchymal stromal cells

Toxicol Sci.

2011

;

122

(

476

488

41.

Chamorro-Garcia

Diaz-Castillo

Shoucri

, et al.

Ancestral perinatal obesogen exposure results in a transgenerational thrifty phenotype in mice

Nat Commun.

2017

;

(

2012

42.

Chamorro-Garca

Sahu

Abbey

Laude

Pham

Blumberg

Transgenerational inheritance of increased fat depot size, stem cell reprogramming, and hepatic steatosis elicited by prenatal exposure to the obesogen tributyltin in mice

Environ Health Perspect.

2013

;

121

(

359

366

43.

Viglietti-Panzica

Panzica

Acute exposure to tributyltin induces c-fos activation in the hypothalamic arcuate nucleus of adult male mice

Neurotoxicology.

2011

;

(

277

280

44.

Penza

Jeremic

Marrazzo

, et al.

The environmental chemical tributyltin chloride (TBT) shows both estrogenic and adipogenic activities in mice which might depend on the exposure dose

Toxicol Appl Pharmacol.

2011

;

255

(

45.

Zuo

Chen

, et al.

Tributyltin causes obesity and hepatic steatosis in male mice

Environ Toxicol.

2011

;

(

46.

Zhang

Chen

Effect of tributyltin on the food intake and brain neuropeptide expression in rats

Endokrynol Pol.

2014

;

(

485

490

47.

Zhang

Sun

Yang

Kong

Zhang

Tributyltin disrupts feeding and energy metabolism in the goldfish (Carassius auratus)

Chemosphere.

2016

;

152

221

228

48.

Riu

McCollum

Pinto

, et al.

Halogenated bisphenol-A analogs act as obesogens in zebrafish larvae (Danio rerio)

Toxicol Sci.

2014

;

139

(

49.

Tingaud-Sequeira

Ouadah

Babin

Zebrafish obesogenic test: a tool for screening molecules that target adiposity

J Lipid Res.

2011

;

(

1765

1772

50.

Rantakokko

Main

Wohlfart-Veje

, et al.

Association of placenta organotin concentrations with growth and ponderal index in 110 newborn boys from Finland during the first 18 months of life: a cohort study

Environ Health.

2014

;

(

51.

Liu

Sun

Lehmler

Bao

Association between urinary tin concentration and diabetes in nationally representative sample of US adults

J Diabetes.

2018

;

(

977

983

52.

Lehmler

Gadogbe

Liu

Bao

Environmental tin exposure in a nationally representative sample of U.S. adults and children: the National Health and Nutrition Examination Survey 2011-2014

Environ Pollut.

2018

;

240

599

606

53.

Gadogbe

Bao

Wels

, et al.

Levels of tin and organotin compounds in human urine samples from Iowa, United States

J Environ Sci Health A Tox Hazard Subst Environ Eng.

2019

;

(

884

890

54.

National Toxicology Program (NTP)

NTP research report on organotin and total tin levels in Danish women of reproductive age. NTP Research Report Series

Research Triangle Park, NC

National Toxicology Program. Research Report 2

;

2016

55.

Liu

Dhana

Furtado

, et al.

Perfluoroalkyl substances and changes in body weight and resting metabolic rate in response to weight-loss diets: a prospective study

PloS Med.

2018

;

(

e1002502

56.

Liu

Rood

, et al.

Perfluoroalkyl substances and changes in bone mineral density: a prospective analysis in the POUNDS-LOST study

Environ Res.

2019

;

179

(

Pt A

108775

57.

Feige

Gelman

Rossi

, et al.

The endocrine disruptor monoethyl-hexyl-phthalate is a selective peroxisome proliferator-activated receptor modulator that promotes adipogenesis

J Biol Chem

2007

;

282

(

19152

19166

58.

Manikkam

Tracey

Guerrero-Bosagna

Skinner

Plastics derived endocrine disruptors (BPA, DEHP and DBP) induce epigenetic transgenerational inheritance of obesity, reproductive disease and sperm epimutations

PloS One.

2013

;

(

e55387

59.

Rochester

Bisphenol A and human health: a review of the literature

Reprod Toxicol.

2013

;

132

155

60.

Miyawaki

Sakayama

Kato

Yamamoto

Masuno

Perinatal and postnatal exposure to bisphenol A increases adipose tissue mass and serum cholesterol level in mice

J Atheroscler Thromb

2007

;

(

245

252

61.

Somm

Schwitzgebel

Toulotte

, et al.

Perinatal exposure to bisphenol A alters early adipogenesis in the rat

Environ Health Perspect.

2009

;

117

(

1549

1555

62.

Le Magueresse-Battistoni

Multigner

Beausoleil

Rousselle

Effects of bisphenol A on metabolism and evidences of a mode of action mediated through endocrine disruption

Mol Cell Endocrinol.

2018

;

475

63.

Alonso-Magdalena

Garca-Arvalo

Quesada

Nadal

Bisphenol-A treatment during pregnancy in mice: a new window of susceptibility for the development of diabetes in mothers later in life

Endocrinology.

2015

;

156

(

1659

1670

64.

Ahmed

Atlas

Bisphenol S- and bisphenol A-induced adipogenesis of murine preadipocytes occurs through direct peroxisome proliferator-activated receptor gamma activation

Int J Obes (Lond).

2016

;

(

1566

1573

65.

Riu

Grimaldi

le Maire

, et al.

Peroxisome proliferator-activated receptor is a target for halogenated analogs of bisphenol A

Environ Health Perspect.

2011

;

119

(

1227

1232

66.

Delfosse

Grimaldi

le Maire

Bourguet

Balaguer

Nuclear receptor profiling of bisphenol-A and its halogenated analogues

Vitam Horm.

2014

;

229

251

67.

Vom Saal

Nagel

Coe

Angle

Taylor

The estrogenic endocrine disrupting chemical bisphenol A (BPA) and obesity

Mol Cell Endocrinol.

2012

;

354

(

1-2

68.

Lukowicz

Ellero-Simatos

Rgnier

, et al.

Metabolic effects of a chronic dietary exposure to a low-dose pesticide cocktail in mice: sexual dimorphism and role of the constitutive androstane receptor

Environ Health Perspect.

2018

;

126

(

067007

69.

Lee

Blumberg

Transgenerational effects of obesogens

Basic Clin Pharmacol Toxicol.

2019

;

125

(

Suppl 3

70.

Casals-Casas

Desvergne

Endocrine disruptors: from endocrine to metabolic disruption

Annu Rev Physiol.

2011

;

135

162

71.

Janesick

Blumberg

Minireview: PPAR as the target of obesogens

J Steroid Biochem Mol Biol.

2011

;

127

(

1-2

72.

Wang

Chao

Tseng

Kuo

Tsou

A recombinant peroxisome proliferator response element-driven luciferase assay for evaluation of potential environmental obesogens

Biotechnol Lett.

2010

;

(

1789

1796

73.

Shoucri

Hung

Chamorro-Garca

Shioda

Blumberg

Retinoid X receptor activation during adipogenesis of female mesenchymal stem cells programs a dysfunctional adipocyte

Endocrinology.

2018

;

159

(

2863

2883

74.

Shoucri

Martinez

Abreo

, et al.

Retinoid X receptor activation alters the chromatin landscape to commit mesenchymal stem cells to the adipose lineage

Endocrinology.

2017

;

158

(

3109

3125

75.

King

Nilsson

Beck

Skinner

Adipocyte epigenetic alterations and potential therapeutic targets in transgenerationally inherited lean and obese phenotypes following ancestral exposures

Adipocyte.

2019

;

(

362

378

76.

Diaz-Castillo

Chamorro-Garcia

Shioda

Blumberg

Transgenerational self-reconstruction of disrupted chromatin organization after exposure to an environmental stressor in mice

Sci Rep.

2019

;

(

13057

77.

Guo

Yan

Cheng

Wei

Kou

Tributyltin exposure induces gut microbiome dysbiosis with increased body weight gain and dyslipidemia in mice

Environ Toxicol Pharmacol.

2018

;

202

208

78.

McLean

Jun

Kozyrskyj

Impact of maternal smoking on the infant gut microbiota and its association with child overweight: a scoping review

World J Pediatr.

2019

;

(

341

349

79.

Wang

Yan

, et al.

Effects of triphenyl phosphate exposure during fetal development on obesity and metabolic dysfunctions in adult mice: impaired lipid metabolism and intestinal dysbiosis

Environ Pollut.

2019

;

246

630

638

80.

Evans

Mangelsdorf

Nuclear receptors, RXR, and the Big Bang

Cell.

2014

;

157

(

255

266

81.

Regnier

El-Hashani

Kamau

Zhang

Massad

Sargis

Tributyltin differentially promotes development of a phenotypically distinct adipocyte

Obesity (Silver Spring).

2015

;

(

1864

1871

82.

Sharma

Staels

Review: peroxisome proliferator-activated receptor gamma and adipose tissueunderstanding obesity-related changes in regulation of lipid and glucose metabolism

J Clin Endocrinol Metab.

2007

;

(

386

395

83.

Kusminski

Bickel

Scherer

Targeting adipose tissue in the treatment of obesity-associated diabetes

Nat Rev Drug Discov.

2016

;

(

639

660

84.

Kim

Monti

Schlezinger

Tributyltin induces a transcriptional response without a brite adipocyte signature in adipocyte models

Arch Toxicol.

2018

;

(

2859

2874

85.

La Merrill

Karey

Moshier

, et al.

Perinatal exposure of mice to the pesticide DDT impairs energy expenditure and metabolism in adult female offspring

PloS One.

2014

;

(

e103337

86.

Turnbaugh

Bckhed

Fulton

Gordon

Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome

Cell Host Microbe.

2008

;

(

213

223

87.

Turnbaugh

Hamady

Yatsunenko

, et al.

A core gut microbiome in obese and lean twins

Nature.

2009

;

457

(

7228

480

484

88.

Vrieze

Van Nood

Holleman

, et al.

Transfer of intestinal microbiota from lean donors increases insulin sensitivity in individuals with metabolic syndrome

Gastroenterology.

2012

;

143

(

913

916.e7

89.

Lai

Chung

Wan

Wong

Bisphenol A alters gut microbiome: Comparative metagenomics analysis

Environ Pollut.

2016

;

218

923

930

90.

Maurice Corinne

Haiser Henry

Turnbaugh Peter

Xenobiotics shape the physiology and gene expression of the active human gut microbiome

Cell

2013

;

152

(

91.

Tousignant

Uno

The effect of obesogens on the microbiota and systemic health in zebrafish

FASEB J

2015

;

(

1 Suppl

850

852

92.

Cross-regulatory circuit between AHR and microbiota

Curr Drug Metab.

2019

;

(

93.

Dou

Duan

Zhang

, et al.

Aryl hydrocarbon receptor (AhR) regulates adipocyte differentiation by assembling CRL4B ubiquitin ligase to target PPAR for proteasomal degradation

J Biol Chem.

2019

;

294

(

18504

18515

94.

Moyer

Rojas

Kerley-Hamilton

, et al.

Obesity and fatty liver are prevented by inhibition of the aryl hydrocarbon receptor in both female and male mice

Nutr Res.

2017

;

95.

Rojas

Moyer

Ringelberg

Tomlinson

CR.

Reversal of obesity and liver steatosis in mice via inhibition of aryl hydrocarbon receptor and altered gene expression of CYP1B1, PPAR, SCD1, and osteopontin [Published online ahead of print January 7, 20200

Int J Obes (Lond)

Doi: 10.1038/s41366-019-0512-z

96.

Wang

Zhang

, et al.

Aryl hydrocarbon receptor deficiency protects mice from diet-induced adiposity and metabolic disorders through increased energy expenditure

Int J Obes (Lond).

2015

;

(

1300

1309

97.

Bock

Aryl hydrocarbon receptor (AHR) functions in NAD+ metabolism, myelopoiesis and obesity

Biochem Pharmacol.

2019

;

163

128

132

98.

Zincker

Lindseth

The Western diet-microbiome-host interaction and its role in metabolic disease

Nutrients

2018

;

(

365

99.

Chassaing

Koren

Goodrich

, et al.

Dietary emulsifiers impact the mouse gut microbiota promoting colitis and metabolic syndrome

Nature.

2015

;

519

(

7541

100.

Janesick

Shioda

Blumberg

Transgenerational inheritance of prenatal obesogen exposure

Mol Cell Endocrinol.

2014

;

398

(

1-2

101.

Skinner

Environmental epigenetics and a unified theory of the molecular aspects of evolution: a neo-Lamarckian concept that facilitates neo-Darwinian evolution

Genome Biol Evol.

2015

;

(

1296

1302

102.

Manikkam

Haque

Guerrero-Bosagna

Nilsson

Skinner

Pesticide methoxychlor promotes the epigenetic transgenerational inheritance of adult-onset disease through the female germline

PloS One.

2014

;

(

e102091

103.

Tracey

Manikkam

Guerrero-Bosagna

Skinner

Hydrocarbons (jet fuel JP-8) induce epigenetic transgenerational inheritance of obesity, reproductive disease and sperm epimutations

Reprod Toxicol.

2013

;

104

116

104.

Skinner

Manikkam

Tracey

Guerrero-Bosagna

Haque

Nilsson

Ancestral dichlorodiphenyltrichloroethane (DDT) exposure promotes epigenetic transgenerational inheritance of obesity

BMC Med.

2013

;

228

105.

Nilsson

Sadler-Riggleman

Skinner

Environmentally induced epigenetic transgenerational inheritance of disease

Environ Epigenet.

2018

;

(

dvy016

106.

Bastos Sales

Kamstra

Cenijn

van Rijt

Hamers

Legler

Effects of endocrine disrupting chemicals on in vitro global DNA methylation and adipocyte differentiation

Toxicol In Vitro.

2013

;

(

1634

1643

107.

Anway

Cupp

Uzumcu

Skinner

Epigenetic transgenerational actions of endocrine disruptors and male fertility

Science.

2005

;

308

(

5727

1466

1469

108.

Nilsson

Skinner

Environmentally induced epigenetic transgenerational inheritance of disease susceptibility

Transl Res.

2015

;

165

(

109.

Hanson

Skinner

Developmental origins of epigenetic transgenerational inheritance

Environ Epigenet

2016

;

(

dvw002

110.

Skinner

Guerrero-Bosagna

Haque

Environmentally induced epigenetic transgenerational inheritance of sperm epimutations promote genetic mutations

Epigenetics.

2015

;

(

762

771

111.

Iqbal

Tran

, et al.

Deleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogramming

Genome Biol.

2015

;

112.

Whitelaw

Disputing Lamarckian epigenetic inheritance in mammals

Genome Biol.

2015

;

113.

Eckersley-Maslin

Alda-Catalinas

Reik

Dynamics of the epigenetic landscape during the maternal-to-zygotic transition

Nat Rev Mol Cell Biol.

2018

;

(

436

450

114.

Heard

Martienssen

Transgenerational epigenetic inheritance: myths and mechanisms

Cell.

2014

;

157

(

109

115.

Saitou

Kagiwada

Kurimoto

Epigenetic reprogramming in mouse pre-implantation development and primordial germ cells

Development.

2012

;

139

(

116.

Ben Maamar

King

Nilsson

Beck

Skinner

Epigenetic transgenerational inheritance of parent-of-origin allelic transmission of outcross pathology and sperm epimutations

Dev Biol.

2020

;

458

(

106

119

117.

Lujn

Caroppo

Niederberger

, et al.

Sperm DNA methylation epimutation biomarkers for male infertility and FSH therapeutic responsiveness

Sci Rep.

2019

;

(

16786

118.

Ben Maamar

Sadler-Riggleman

Beck

Skinner

Epigenetic transgenerational inheritance of altered sperm histone retention sites

Sci Rep.

2018

;

(

5308

119.

Skinner

Ben Maamar

Sadler-Riggleman

, et al.

Alterations in sperm DNA methylation, non-coding RNA and histone retention associate with DDT-induced epigenetic transgenerational inheritance of disease

Epigenetics Chromatin.

2018

;

(

120.

Muncke

Endocrine disrupting chemicals and other substances of concern in food contact materials: an updated review of exposure, effect and risk assessment

J Steroid Biochem Mol Biol.

2011

;

127

(

1-2

118

127

121.

Fristachi

Rice

Impellitteri

Carlson-Lynch

Little

Using probabilistic modeling to evaluate human exposure to organotin in drinking water transported by polyvinyl chloride pipe

Risk Anal.

2009

;

(

1615

1628

122.

Milton

Lacerda

Sinoti

SBP

, et al.

Dibutyltin compounds effects on PPAR/RXR activity, adipogenesis, and inflammation in mammalians cells

Front Pharmacol.

2017

;

507

123.

Chamorro-Garca

Shoucri

Willner

Kch

Janesick

Blumberg

Effects of perinatal exposure to dibutyltin chloride on fat and glucose metabolism in mice, and molecular mechanisms, in vitro

Environ Health Perspect.

2018

;

126

(

057006

124.

Liu

Lehmler

Sun

, et al.

Bisphenol A substitutes and obesity in US adults: analysis of a population-based, cross-sectional study

Lancet Planet Health.

2017

;

(

e114

e122

125.

Pelch

Wignall

Goldstone

, et al.

A scoping review of the health and toxicological activity of bisphenol A (BPA) structural analogues and functional alternatives

Toxicology.

2019

;

424

152235

126.

Ivry Del Moral

Le Corre

Poirier

, et al.

Obesogen effects after perinatal exposure of 4,4-sulfonyldiphenol (bisphenol S) in C57BL/6 mice

Toxicology.

2016

;

357-358

127.

Jacobson

Woodward

Bao

Liu

Trasande

Urinary bisphenols and obesity prevalence among U.S. children and adolescents

J Endocr Soc.

2019

;

(

1715

1726

128.

Ruiz

Faroon

Mumtaz

, et al.;

Agency for Toxic Substances and Disease Registry (ATSDR)

Toxicological profile for Acrylamide

Atlanta, GA

US Department of Health and Human Services, Public Health Service

;

2012

https://stacks.cdc.gov/view/cdc/20859129.

Lee

Pyo

Acrylamide induces adipocyte differentiation and obesity in mice

Chem Biol Interact.

2019

;

298

130.

Kadawathagedara

Tong

ACH

Heude

, et al.

Dietary acrylamide intake during pregnancy and anthropometry at birth in the French EDEN Mother-Child Cohort Study

Environ Res.

2016

;

149

189

196

131.

Kadawathagedara

Botton

de Lauzon-Guillain

, et al.

Dietary acrylamide intake during pregnancy and postnatal growth and obesity: results from the Norwegian Mother and Child Cohort Study (MoBa)

Environ Int.

2018

;

113

325

334

132.

Huang

Zhuang

Jiao

Wang

Zhang

Association of acrylamide hemoglobin biomarkers with obesity, abdominal obesity and overweight in general US population: NHANES 2003-2006

Sci Total Environ

2018

;

631632

589

596

133.

Chu

Lin

Chen

Lin

Negative association between acrylamide exposure and body composition in adults: NHANES, 2003-2004

Nutr Diabetes.

2017

;

(

e246

134.

Gray

Kanagy

Furlong

, et al.

Presence of the Corexit component dioctyl sodium sulfosuccinate in Gulf of Mexico waters after the 2010 Deepwater Horizon oil spill

Chemosphere.

2014

;

124

130

135.

Temkin

Bowers

Magaletta

, et al.

Effects of crude oil/dispersant mixture and dispersant components on PPAR activity in vitro and in vivo: identification of dioctyl sodium sulfosuccinate (DOSS; CAS #577-11-7) as a probable obesogen

Environ Health Perspect.

2016

;

124

(

112

119

136.

Temkin

Bowers

Ulmer

, et al.

Increased adiposity, inflammation, metabolic disruption and dyslipidemia in adult male offspring of DOSS treated C57BL/6 dams

Sci Rep.

2019

;

(

1530

137.

Bowers

Temkin

Guillette

Baatz

Spyropoulos

The commonly used nonionic surfactant Span 80 has RXR transactivation activity, which likely increases the obesogenic potential of oil dispersants and food emulsifiers

Gen Comp Endocrinol.

2016

;

238

138.

Sun

Yang

Liu

, et al.

Butylated hydroxyanisole isomers induce distinct adipogenesis in 3T3-L1 cells

J Hazard Mater.

2019

;

379

120794

139.

Sun

Tang

Yang

, et al.

Perturbation of 3-tert-butyl-4-hydroxyanisole in adipogenesis of male mice with normal and high fat diets

Sci Total Environ.

2020

;

703

135608

140.

Matsuyama

Oki

Yokoki

Obesity induced by monosodium glutamate in mice

Natl Inst Anim Health Q (Tokyo).

1973

;

(

101

141.

Shannon

Green

Willars

, et al.

The endocrine disrupting potential of monosodium glutamate (MSG) on secretion of the glucagon-like peptide-1 (GLP-1) gut hormone and GLP-1 receptor interaction

Toxicol Lett.

2017

;

265

105

142.

Shannon

Wilson

Xie

Connolly

In vitro bioassay investigations of suspected obesogen monosodium glutamate at the level of nuclear receptor binding and steroidogenesis

Toxicol Lett.

2019

;

301

143.

Ren

X-M

Blumberg

Agrochemicals and obesity

Mol Cell Endocrinol

2020

;in press.

144.

Warner

Harley

Kogut

Bradman

Eskenazi

Prenatal DDT exposure and child adiposity at age 12: the CHAMACOS study

Environ Res.

2017

;

159

606

612

145.

Valvi

Mendez

Martinez

, et al.

Prenatal concentrations of polychlorinated biphenyls, DDE, and DDT and overweight in children: a prospective birth cohort study

Environ Health Perspect.

2012

;

120

(

451

457

146.

Park

Kim

, et al.

Imidacloprid, a neonicotinoid insecticide, potentiates adipogenesis in 3T3-L1 adipocytes

J Agric Food Chem.

2013

;

(

255

259

147.

Sun

Xiao

Kim

, et al.

Imidacloprid promotes high fat diet-induced adiposity and insulin resistance in male C57BL/6J mice

J Agric Food Chem.

2016

;

(

9293

9306

148.

Kubsad

Nilsson

King

Sadler-Riggleman

Beck

Skinner

Assessment of glyphosate induced epigenetic transgenerational inheritance of pathologies and sperm epimutations: generational toxicology

Sci Rep.

2019

;

(

6372

149.

Biserni

Mesnage

Ferro

, et al.

Quizalofop-p-ethyl induces adipogenesis in 3T3-L1 adipocytes

Toxicol Sci.

2019

;

170

(

452

461

150.

Attina

Hauser

Sathyanarayana

, et al.

Exposure to endocrine-disrupting chemicals in the USA: a population-based disease burden and cost analysis

Lancet Diabetes Endocrinol.

2016

;

(

996

1003

151.

Legler

Fletcher

Govarts

, et al.

Obesity, diabetes, and associated costs of exposure to endocrine-disrupting chemicals in the European Union

J Clin Endocrinol Metab.

2015

;

100

(

1278

1288

152.

Newbold

Padilla-Banks

Snyder

Jefferson

Developmental exposure to estrogenic compounds and obesity

Birth Defects Res A Clin Mol Teratol.

2005

;

(

478

480

153.

Kuznetsova

Wang

Rao

, et al.

Glucocorticoid receptor and nuclear factor kappa-b affect three-dimensional chromatin organization

Genome Biol.

2015

;

264

154.

Trotter

Archer

Nuclear receptors and chromatin remodeling machinery

Mol Cell Endocrinol.

2007

;

265-266

162

167

155.

Qin

Roberts

Grimm

, et al.

An obesity-associated gut microbiome reprograms the intestinal epigenome and leads to altered colonic gene expression

Genome Biol.

2018

;

(

156.

Paul

Barnes

Demark-Wahnefried

, et al.

Influences of diet and the gut microbiome on epigenetic modulation in cancer and other diseases

Clin Epigenetics.

2015

;

112

157.

Jacobsen

Axelstad

Boberg

, et al.

Persistent developmental toxicity in rat offspring after low dose exposure to a mixture of endocrine disrupting pesticides

Reprod Toxicol.

2012

;

(

237

250

158.

Kjaerstad

Taxvig

Andersen

Nellemann

Mixture effects of endocrine disrupting compounds in vitro

Int J Androl.

2010

;

(

425

433

159.

Kortenkamp

Low dose mixture effects of endocrine disrupters: implications for risk assessment and epidemiology

Int J Androl.

2008

;

(

233

240

160.

Le Magueresse-Battistoni

Vidal

Naville

Environmental pollutants and metabolic disorders: the multi-exposure scenario of life

Front Endocrinol (Lausanne).

2018

;

582

Endocrine Society 2020.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (

http://creativecommons.org/licenses/by/4.0/

), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

The Environmental Impact of Dachshund Obesity

The Environmental Foodprint of Obesity

Abstract

MeSH terms

Substances

Environmental Obesogens and Their Impact on Susceptibility to Obesity: New Mechanisms and Chemicals

Abstract

The Obesogen Hypothesis

Classic Effects and Mechanisms of Obesogens

Mechanisms of Obesogen Action: Beyond Peroxisome ProliferatorActivated Receptor

Adipocyte commitment

Adipocyte health

Gut microbiome dysbiosis

Possible Mechanisms Underlying Transgenerational Effects of Obesogen Exposure

Epigenetic modifications

Chromatin accessibility

Newly Discovered Obesogens (New Threats)

Dibutyltin

Bisphenol A analogues

Acrylamide

Surfactants

Food additives

Pesticides

Future Directions

Abbreviations

Acknowledgment

Additional Information

References

Another articles related to The Environmental Impact of Dachshund Obesity